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Dye-loaded fluorescent polymeric nanoparticles: bright platform for biosensing and bioimaging 
Thursday, 12 July 2018,  4:00
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Dye-loaded fluorescent polymeric nanoparticles: bright platform for biosensing and bioimaging

Andrey S. Klymchenko

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Faculté de Pharmacie, ILLKIRCH, France


Short abstract:

Fluorescent polymeric nanoparticles (NPs) appear as an attractive alternative to inorganic NPs, such as quantum dots
(QDs), because of their potential biodegradability, low toxicity and high capacity to encapsulate active molecules.[1]
Important current challenges with organic NPs are (i) achieving high fluorescence brightness for the smallest possible size
and (ii) realizing efficient Förster resonance energy transfer (FRET) from NPs, which are normally larger than the Förster
radius. We showed that the small size of NPs can be achieved by using nanoprecipitation of polymers bearing 1-2 charged
groups per chain.[2,3] To achieve high NPs brightness, we designed cationic dyes (rhodamines and cyanines) containing
large fluorinated counterions that prevent aggregation caused self-quenching of dyes (Figure 1).[2,4] As a result, we
obtained PLGA and PMMA NPs of 15-60 nm encapsulating 100-10000 of dyes, which are many-fold brighter than QDs.
Moreover, inside NPs, dyes showed remarkable collective behavior, observed as particle on/off switching, due to ultrafast
exciton migration.[2,5] This collective behavior of dyes was successfully applied to obtain giant light-harvesting
nanoantenna that amplifies ~1000-fold emission of single FRET acceptor dyes and enables first single-molecule detection
in ambient sunlight conditions.[5] This organic nanoantenna was successfully applied for amplified fluorescence detection
of biomolecules. Moreover, using NPs encapsulating cyanine dyes, we developed new methodology for long-term color
coding of living cells in vitro and in vivo.[6]


[1] Reisch, A.; Klymchenko, A.S. Small, 2016, 12 , 1968.
[2] Reisch, A.; Didier, P.; Richert, L.; Oncul, S.; Arntz, Y.; Mely, Y.; Klymchenko, A. S. Nat. Commun. 2014, 5, 4089.
[3] Reisch, A.; Runser, A.; Arntz, Y.; Mély, Y.; Klymchenko, A.S. ACS Nano, 2015, 9, 5104.
[4] Shulov, I.; Oncul, S.; Reisch, A.; Arntz, Y.; Collot, M.; Mely, Y.; Klymchenko, A. S. Nanoscale, 2015, 7, 18198.
[5] Trofymchuk, K., Reisch, A., Didier, P. Fras, F., Gilliot, P. Mely, Y. Klymchenko, AS, Nature Photonics, 2017, 11, 657.
[6] Andreiuk B, Reisch A, Lindecker M, Follain G, Peyriéras N, Goetz JG, Klymchenko AS, Small, 2017, 13, 1701582.

Location ICN2 Seminar Room
Contact This email address is being protected from spambots. You need JavaScript enabled to view it.



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